Brain adenosine 5′-triphosphate–creatine phosphotransferase. Purification, thiol group reactivity and the amino acid sequence around the reactive thiol groups
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Amino acid sequence around the thiol and reactive acyl groups ofhuman
Activation of the fourth component of complement (C4) by C I s results in the generation of a reactive acyl group, able to react with putrescine, and in the release of a free thiol group that cannot be detected in the native haemolytically active molecule. Both the reactive acyl group and the free thiol group have been shown to reside in C4d, a fragment of the a'-chain of C4b derived from diges...
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The yellow colored sulfhydryl reagent, iV-(4-dimethylamino-3,!%dinitrophenyl)maleimide, was used in the isolation of the peptide containing the reactive sulfhydryl group of chymopapain. Titration of cyanide-activated chymopapain B with p-chloromercuribenzoate at pH 4.6 indicated that there is a maximum of 1.4 moles of sulfhydryl per mole of the enzyme. Alkylation of half of the p-chloromercurib...
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1. A column procedure for the purification of creatine kinase from normal and dystrophic mouse muscle is described. 2. The native enzymes are indistinguishable by various physical criteria and have mol.wt. about 80000. 3. The purified enzyme from dystrophic muscle is only half as active as the normal, contains only one thiol group readily alkylated by iodoacetamide instead of two and has one le...
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1. Considerable differences were found between the rates and degrees of modification of native trout actin with iodo[2-(14)C]acetate and iodo[1-(14)C]acetamide. 2. With iodoacetate, G- and F-actin were both labelled in the N-terminal peptide only. This modification had little effect on the ability of the actin to polymerize. 3. Iodoacetamide labelled three cysteine residues in both G- and F-act...
متن کاملThe reactivity of the thiol groups of calf thymus deoxyribonucleohistone.
The reactivities of the two cysteine thiol groups of calf thymus F3 histone were investigated using 5,5'-dithiobis-[2- nitrobenzoic acid], (DTNB). In isolated histone, both thiol groups were available for reaction. However, analysis of reaction profiles of native deoxyribonucleohistone, (DNH), in various solvent conditions, together with gel electrophoresis studies of DNH modified with DTNB, sh...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1970
ISSN: 0306-3283
DOI: 10.1042/bj1200589